Genotoxic and cytotoxic effects of the drug dipyrone sodium in African green monkey kidney (Vero) cell line exposed in vitro

Genotoxic and cytotoxic effects of the drug dipyrone sodium in African green monkey kidney (Vero) cell line exposed in vitro

Genotoxic and cytotoxic effects of the drug dipyrone sodium in African green monkey kidney (Vero) cell line exposed in vitro

Dipyrone or metamizole is likely one of the most used analgesics, primarily resulting from its low monetary price. Nevertheless, in some international locations, the sale of dipyrone is prohibited resulting from reported extreme circumstances of agranulocytosis because of its use. Regardless of its excessive use, research displaying genotoxic and cytotoxic results of dipyrone in mammalian cells are scarce.

Subsequently, within the current examine, we assessed cell viability, genotoxic results, cytotoxic results (by apoptosis and necrosis induction), and the induction of reactive oxygen species (ROS) in Vero cells (a cell line obtained from the pink kidney of inexperienced monkey) uncovered to dipyrone.

Our outcomes confirmed a major discount in viability of cells uncovered to dipyrone by the MTT assay. A big improve in injury index evaluated by a comet assay was additionally noticed, which signifies its genotoxic results. Wherein issues the cytotoxic results of dipyrone, we noticed a major improve within the variety of apoptotic cells utilizing fluorescent dyes after 24 h and 48 h of remedy with the drug.

Our outcomes additionally confirmed that there was no vital distinction within the induction of ROS era after remedy of the cells with the drug assessed by the DCFH-DA assay. Thus, our work confirmed that dipyrone is each a genotoxic and cytotoxic drug to Vero cells within the assessed situations.

The mucus integrity and irregular inflammatory response are the essential biomarker of inflammatory bowel illness (IBD). Velvet antler (VA) has been used as conventional Chinese language medicines for a few years. Anti-inflammatory property was demonstrated by way of suppression of cyclooxygenase-2 and cytokines protein expression. And it has additional proved to advertise wound therapeutic in streptozotocin-induced diabetic rats mannequin.

The aforementioned functionalities of VA extracts could also be related to the remedy of IBD. Thus, the intention of current examine was to guage the impact of velvet antler water extracts kind Formosan Sambar deer (Rusa unicolor swinhoei, SVAE) and pink deer (Cervus elaphus, RVAE) on the barrier perform and to analyze the potential mechanism utilizing in vitro mannequin.

Human colonic epithelial cell fashions (Caco-2) had been co-cultured with numerous concentrations of each SVAE and RVAE (250-500 µg mL-1) in dextran sulfate sodium (DSS)-induced colitis mannequin. Trans-epithelial electrical resistance (TEER) worth and the macromolecule permeability of Fluorescein isothiocyanate (FITC)-labeled dextran had been measured to guage the integrity of monolayer of Caco-2.

Western blotting was carried out for evaluation of protein expressions of occludin, Zonula occludens-1 (ZO-1), claudin-1, claudin-2 and myosin gentle chain kinase (MLCK). The cytotoxicity was performed by MTT assay.

Security of Pink Blood Cell Transfusion Utilizing Small Central Strains in Neonates: An in vitro Non-inferiority Examine

This examine aimed to analyze the security of transfusing pink blood cell concentrates (RBCCs) by way of small [24 gauge (24G)] and extra-small [28 gauge [28G)] peripherally inserted central catheters (PICCs), in line with pointers of transfusion observe in Switzerland. We carried out a non-inferiority in vitro examine to evaluate the security of transfusing RBCC for four h at a four ml/h pace by way of 24G silicone and 28G polyurethane PICC traces, in contrast with a peripheral 24G brief catheter.

The first endpoint was hemolysis share. Secondary endpoints had been catheter occlusion, inline stress, and potassium and lactate values. For the first consequence, hemolysis values weren’t statistically completely different amongst catheter teams (0.06% variation, p = 0.95) or over time (2.75% variation, p = 0.72). The best hemolysis values in each 24G and 28G PICCs had been beneath the non-inferiority predefined margin.

We didn’t observe catheter occlusion. Inline stress diversified between catheters however adopted the identical sample of speedy improve adopted by stabilization. Potassium and lactate measurements weren’t statistically completely different amongst examined catheters (0.139% variation, p = 0.98 for potassium and 0.062%, p = 0.96 for lactates).

This examine exhibits that RBCC transfusion carried out in vitro by way of 24G silicone and 28G polyurethane PICC traces is possible with out detectable hemolysis or stress issues. Additionally, it provides that, regarding hemolysis, transfusion of RBCC in small and extra-small PICC traces is non-inferior to peripheral brief 24G catheters. Scientific potential evaluation in preterm infants is required to verify these knowledge additional.

Genotoxic and cytotoxic effects of the drug dipyrone sodium in African green monkey kidney (Vero) cell line exposed in vitro

Affect of Piper betle L. extract on umbilical twine cells in vitro and potential treating cutaneous wound

This analysis aimed to check the consequences of Piper betle L. from Vietnam on fibroblasts and UC-derived mesenchymal stem cells (UC-MSCs) from human umbilical twine (UC) on the scratch assay. We examined the extract at completely different concentrations after which assessed the extent of expression of the elements concerned within the inflammatory course of on fibroblasts together with IL-33, VCAM, CD248 by assay actual time qPCR.

On the concentrations of 0.025 μL/mL and 0.03 μL/mL, the extracts positively affected fibroblast proliferation and UC-MSCs. In contrast, the focus of 0.058 μL/mL, the extract was poisonous to UC-MSCs and fibroblast cell traces, the cells had been now not in a position to survive. qPCR outcomes present that Piper betle L. extract has the flexibility to scale back the expression ranges of IL-33 (50.8%)

Vitronectin antibody
10-1962 200 ul
EUR 543
Description: Mouse monoclonal Vitronectin antibody

Vitronectin antibody
10-1963 200 ul
EUR 543
Description: Mouse monoclonal Vitronectin antibody

Vitronectin antibody
10-1964 200 ul
EUR 769
Description: Mouse monoclonal Vitronectin antibody

Vitronectin antibody
10-1965 200 ul
EUR 543
Description: Mouse monoclonal Vitronectin antibody

Vitronectin antibody
10R-8488 100 ul
EUR 393
Description: Mouse monoclonal Vitronectin antibody

Vitronectin Antibody
48876-100ul 100ul
EUR 333

Vitronectin Antibody
48876-50ul 50ul
EUR 239

Vitronectin antibody
70R-50548 100 ul
EUR 244
Description: Purified Polyclonal Vitronectin antibody

Vitronectin Antibody
abx023996-200ug 200 ug
EUR 578
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Vitronectin antibody (HRP)
60R-1036 100 ug
EUR 358
Description: Sheep polyclonal Vitronectin antibody (HRP) conjugated

Human Vitronectin Antibody
48629-05011 150 ug
EUR 217

Vitronectin (VTN) Antibody
20-abx008574
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Vitronectin (VTN) Antibody
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Vitronectin (VTN) Antibody
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Vitronectin (VTN) Antibody
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Vitronectin (VTN) Antibody
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Vitronectin (VTN) Antibody
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Vitronectin (VTN) Antibody
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EUR 286
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abx239415-100ug 100 ug
EUR 509
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Vitronectin Conjugated Antibody
C48876 100ul
EUR 397

Vitronectin (VTN) Antibody
20-abx339876
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Vitronectin (VTN) Antibody
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20-abx320309
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Vitronectin (VTN) Antibody
20-abx327315
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Vitronectin (VTN) Antibody
20-abx318002
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Vitronectin (VTN) Antibody
20-abx321678
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Vitronectin Polyclonal Antibody
ABP57393-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from the Internal region of human Vitronectin
  • Applications tips:
Description: A polyclonal antibody for detection of Vitronectin from Human. This Vitronectin antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Vitronectin

Vitronectin Polyclonal Antibody
ABP57393-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from the Internal region of human Vitronectin
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Description: A polyclonal antibody for detection of Vitronectin from Human. This Vitronectin antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Vitronectin

Vitronectin Polyclonal Antibody
ABP57393-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from the Internal region of human Vitronectin
  • Applications tips:
Description: A polyclonal antibody for detection of Vitronectin from Human. This Vitronectin antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Vitronectin

Vitronectin Polyclonal Antibody
ABP56513-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from the Internal region of human Vitronectin
  • Applications tips:
Description: A polyclonal antibody for detection of Vitronectin from Human, Mouse, Rat. This Vitronectin antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Vitronectin

Vitronectin Polyclonal Antibody
ABP56513-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from the Internal region of human Vitronectin
  • Applications tips:
Description: A polyclonal antibody for detection of Vitronectin from Human, Mouse, Rat. This Vitronectin antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Vitronectin

Vitronectin Polyclonal Antibody
ABP56513-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from the Internal region of human Vitronectin
  • Applications tips:
Description: A polyclonal antibody for detection of Vitronectin from Human, Mouse, Rat. This Vitronectin antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Vitronectin

Vitronectin (VTN) Antibody
20-abx001401
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
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Vitronectin Polyclonal Antibody
ES7512-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against Vitronectin from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

Vitronectin Polyclonal Antibody
ES7512-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against Vitronectin from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

Vitronectin Polyclonal Antibody
ES8386-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against Vitronectin from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

Vitronectin Polyclonal Antibody
ES8386-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against Vitronectin from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

anti- Vitronectin antibody
FNab09415 100µg
EUR 548.75
  • Recommended dilution: WB: 1:500-1:2000
  • IHC: 1:50-1:200
  • Immunogen: vitronectin
  • Uniprot ID: P04004
  • Gene ID: 7448
  • Research Area: Immunology, Cardiovascular, Signal Transduction
Description: Antibody raised against Vitronectin

Anti-Vitronectin antibody
PAab09415 100 ug
EUR 386

Anti-Vitronectin antibody
STJ16100270 1 mL
EUR 720

Anti-Vitronectin antibody
STJ16100802 100 µg
EUR 506

Anti-Vitronectin antibody
STJ96249 200 µl
EUR 197
Description: Rabbit polyclonal to Vitronectin.

Anti-Vitronectin antibody
STJ97659 200 µl
EUR 197
Description: Rabbit polyclonal to Vitronectin.

Vitronectin protein
30R-2452 100 ug
EUR 570
Description: Purified native Human Vitronectin protein

Vitronectin Protein
abx069978-100ug 100 ug
EUR 739
  • Shipped within 5-10 working days.

Vitronectin/VTN
E21-395 10ug
EUR 343

Human Vitronectin
AK9773-0100 100µg Ask for price

Human Vitronectin
AK9773-0500 500µg Ask for price

anti-Vitronectin
YF-PA15295 50 ul
EUR 363
Description: Mouse polyclonal to Vitronectin

anti-Vitronectin
YF-PA15296 50 ug
EUR 363
Description: Mouse polyclonal to Vitronectin

anti-Vitronectin
YF-PA15297 100 ul
EUR 403
Description: Rabbit polyclonal to Vitronectin

anti-Vitronectin
YF-PA15298 100 ug
EUR 403
Description: Rabbit polyclonal to Vitronectin

Polyclonal VTN / Vitronectin Antibody
APS00018G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Sheep that recognizes and binds to Human VTN / Vitronectin . This antibody is tested and proven to work in the following applications:

Vitronectin (VTN) Antibody (Biotin)
20-abx272081
  • EUR 439.00
  • EUR 244.00
  • EUR 1261.00
  • EUR 606.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
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  • Shipped within 5-15 working days.

Vitronectin (VTN) Antibody Pair
20-abx370512
  • EUR 1790.00
  • EUR 1149.00
  • 10 × 96 tests
  • 5 × 96 tests
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Vitronectin (VTN) Antibody (HRP)
20-abx313184
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
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  • 50 ug
  • Shipped within 5-10 working days.

Vitronectin (VTN) Antibody (FITC)
20-abx313185
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
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  • Shipped within 5-10 working days.

Vitronectin (VTN) Antibody (Biotin)
20-abx313186
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Monoclonal VTN / Vitronectin Antibody
AMM02177G 0.05ml
EUR 484
Description: A Monoclonal antibody against Human VTN / Vitronectin. The antibodies are raised in Mouse. This antibody is applicable in WB and IHC-P, E

Anti-Vitronectin/VTN Antibody
PA1799 100ug/vial
EUR 294

Anti-Vitronectin/VTN Antibody
PB9458 100ug/vial
EUR 334

Human Vitronectin Antibody (Biotin Conjugate)
48629-05021 150 ug
EUR 276

Anti-Vitronectin Monoclonal Antibody (BV1)
CTA-191-100ug 100ug Ask for price
Description: Mouse anti-Vitronectin monoclonal antibody (BV1) for WB, Flow, IP.

Anti-Vitronectin Monoclonal Antibody (BV1)
CTA-191-1mg 1mg Ask for price
Description: Mouse anti-Vitronectin monoclonal antibody (BV1) for WB, Flow, IP.

Rabbit Anti-Vitronectin Polyclonal Antibody
CTA-430-100ug 100ug Ask for price
Description: Rabbit anti-Vitronectin polyclonal antibodyfor ICC/IF, IHC, IHC-P.

Rabbit Anti-Vitronectin Polyclonal Antibody
CTA-430-1mg 1mg Ask for price
Description: Rabbit anti-Vitronectin polyclonal antibodyfor ICC/IF, IHC, IHC-P.

Goat Anti-Vitronectin Polyclonal Antibody
CTA-431-100ug 100ug Ask for price
Description: Goat anti-Vitronectin polyclonal antibodyfor WB, Simple Western, IHC.

Goat Anti-Vitronectin Polyclonal Antibody
CTA-431-1mg 1mg Ask for price
Description: Goat anti-Vitronectin polyclonal antibodyfor WB, Simple Western, IHC.

Anti-Vitronectin Rabbit Monoclonal Antibody
M02271 100ug/vial
EUR 397
Description: Rabbit Monoclonal Vitronectin Antibody. Validated in IF, WB and tested in Human, Mouse, Rat.

Vitronectin (VTN) Polyclonal Antibody (Human)
4-PAB041Hu02
  • EUR 239.00
  • EUR 2391.00
  • EUR 598.00
  • EUR 299.00
  • EUR 211.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: VTN (Thr400~Tyr468)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Vitronectin (VTN)

Vitronectin (VTN) Polyclonal Antibody (Human)
4-PAB041Hu03
  • EUR 247.00
  • EUR 2510.00
  • EUR 625.00
  • EUR 310.00
  • EUR 214.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Ala19~Tyr468
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Vitronectin (VTN)

Vitronectin (VTN) Polyclonal Antibody (Mouse)
4-PAB041Mu01
  • EUR 243.00
  • EUR 2450.00
  • EUR 611.00
  • EUR 304.00
  • EUR 212.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Pro250~Lys478
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Vitronectin (VTN)

Vitronectin (VTN) Polyclonal Antibody (Rat)
4-PAB041Ra01
  • EUR 251.00
  • EUR 2589.00
  • EUR 643.00
  • EUR 317.00
  • EUR 216.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: VTN (Gln21~Arg400)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rat Vitronectin (VTN)

Vitronectin, Human Plasma
4087-100
EUR 457

Vitronectin Blocking Peptide
20-abx061910
  • EUR 258.00
  • EUR 384.00
  • 1 mg
  • 5 mg
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Vitronectin Blocking Peptide
20-abx063423
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg
  • Shipped within 5-10 working days.

Vitronectin (VTN) Protein
20-abx262778
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

anti-Vitronectin (10E12)
LF-MA0205 100 ul
EUR 334
Description: Mouse monoclonal to Vitronectin

Recombinant Vitronectin (VTN)
4-RPB041Hu01
  • EUR 476.32
  • EUR 230.00
  • EUR 1511.20
  • EUR 570.40
  • EUR 1040.80
  • EUR 382.00
  • EUR 3628.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: P04004
  • Buffer composition: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 11.6kDa
  • Isoelectric Point: 4.7
Description: Recombinant Human Vitronectin expressed in: E.coli

Recombinant Vitronectin (VTN)
4-RPB041Hu02
  • EUR 476.32
  • EUR 230.00
  • EUR 1511.20
  • EUR 570.40
  • EUR 1040.80
  • EUR 382.00
  • EUR 3628.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: P04004
  • Buffer composition: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 9.4kDa
  • Isoelectric Point: 6
Description: Recombinant Human Vitronectin expressed in: E.coli

Recombinant Vitronectin (VTN)
4-RPB041Hu03
  • EUR 476.32
  • EUR 230.00
  • EUR 1511.20
  • EUR 570.40
  • EUR 1040.80
  • EUR 382.00
  • EUR 3628.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: P04004
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 55.0kDa
  • Isoelectric Point: 5.4
Description: Recombinant Human Vitronectin expressed in: E.coli

VCAM (32.1%), CD248 (46.13%) which set off inflammatory processes, thereby decreasing mobile stress and selling the method of therapeutic scratches. Our examine revealed the proliferation capabilities of Piper betle L. extract from Vietnam In vitro. Therefore, Piper betle L. may very well be really useful as a possible supply of wound therapeutic brokers.

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